The Vice Squad officers appear, and begin to chase, the player upon reaching a three-star wanted level. Killing a Vice Squad member will cause them to drop their Micro SMG as well as two wads of cash. The Vice Squad members are the only law enforcement members who drop money upon being killed. The Vice Squad members always arrive as two unaided officers, dressed casually, but tastefully.
In Vice City, there are six Vice Squad officers, only two of which are selected randomly when chasing the player. While in Vice City Stories, there are only two Vice Squad officers, one is Caucasian and the other is African-American.
The Vice Squad members come in pairs and drive VCPD Cheetahs of varying colours which are fast and can easily catch up with the player on foot or in a car. The VCPD Cheetah is the only emergency vehicle that can be resprayed in a Pay 'n' Spray and/or a Spray 'n' Go. However, it cannot be resprayed in Grand Theft Auto: Vice City Stories.
The Vice Squad members carry Micro SMGs as their main choice of weapon.
- The Vice Squad members' looks in Grand Theft Auto: Vice City are inspired by leading characters from Miami Vice, a legendary television series from the 1980s focusing on well-dressed vice officers in Miami, who drive expensive sports cars and live a life of adventure.
- There is a white officer with dirty blond hair - a reference to Sonny Crockett
- There is a black officer - a reference to Rico Tubbs.
- There is a dark haired, bearded officer - a reference to Larry Zito.
- There is an overweight white officer - a reference to Stan Switek.
- There is an officer wearing a formal suit - a reference to Lt. Marty Castillo.
- The VCPD Cheetah, Vice Squad's patrol vehicle, is based on the 1986 Ferrari Testarossa that Sonny Crockett drives Season 3 onwards.
- The Vice Squad characters in the multiplayer component of Grand Theft Auto: Vice City Stories are named "Officer Cracker" and "Officer Butts", a crude take on the names of Sonny Crockett and Rico Tubbs.
- The Vice Squad was mentioned once in Grand Theft Auto: Liberty City Stories; during the mission "Salvatore's Salvation", JD O'Toole says, "But you know Salvatore, he's gonna get the Vice Squad on my ass."
- The "Vice Squad" graffiti can be seen on the walls all over Liberty City in Grand Theft Auto IV.