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Not long after dropping off the Tanker full of unusual chemicals at a warehouse, the protagonist is contacted by El Burro who says that the guys who offloaded the chemicals have developed some sort of disease and must be disposed of before the police pick them up and find out about the chemicals. The first target is Pepe, who is walking in central Eagleside. After killing him, the protagonist must head to northwest Eagleside (although the dialogue says Potato) and find Jorge, who is driving around in his blue Portsmouth. He must be intercepted and killed also. The next target is Jose, who is just leaving a bar in east Woodside. After killing Jose, the protagonist must then head to northwest Sunrise and find Pedro, who is attempting to leave the city in his blue Portsmouth and must be dealt with in the same way as Jorge. Alfonso is the next target and can be located in northwest Atlantic Heights, on his way to the Red Clam Bar. After killing Alfonso, the protagonist must head to southeast Market and locate El Burro's oldest compadre Rodrigo, who is driving around in his blue Portsmouth. When he is intercepted, Rodrigo will shoot back at the protagonist and must be killed quickly. The last of El Burro's men to be disposed of is Chico who can be found in northwest Woodside; killing him will complete the mission.
The player is awarded 50,000 points and a +1 score multiplier for completing the mission.
- If the player failed Tequila Slammer - Phone 4: Mission 1, the introduction for this mission is different. El Burro will say other of his men, Jefe, managed to collect another truck containing the same chemicals.
- There is no time limit to accomplish the task, despite the urging in some of El Burro's messages. The player can drive to a respray shop between two murders, the target persons won't escape.